Protein data bank and nucleic acids data bank

For this post, we will learn about how to search for article and gene through the website for National Center for Biotechnology Information (NCBI) www.ncbi.nlm.nih.gov :


First, we need to know the following in the website:
  • Nucleotide : the nucleotide database is a collection of sequences from..
  • Blast : Protein BLAST - Blastx - Tblastn - Recent Results - Tblastx - ...
  • Gene : A portal to gene-specific content based on NCBI's RefSeq project ...
  • PubMed : PubMed comprises more than 25 million citations for biomedical ...
  • DNA & RNA : GenBank -Nucleotide - Assembly - BioSample - ...

HOW TO SEARCH FOR ARTICLES
To search for particular articles using PubMed in the website precisely, we need to pay attention to what keywords we use. Using different keywords, different types of article will appear in search results in PubMed; for example, certain keywords will lead to certain results such as :


To search for articles in a specific field, the following tags are used:
MeSH Headings [MH]
Afflitiation [AD]
All Fields [ALL]
Author [AU]
Book [book]
Editor [ED]
First Author Name [1AU]
ISBN [ISBN]
Issue [IP]
Last Author [LASTAU]
Publication Date [DP]
Publication Type [PT]
Text Words [TW]
Title [TI]

HOW TO SEARCH GENE
For example:
"ABO transferase A" AND human[orgn]
"Blood type"

F2 AND "coagulation factor II"

Result:

DNA and protein have different sequences. To differentiate them, DNA sequences contain only four letters - G, A, T, and C , as shown in the above figure.
While protein sequences use 20 letters, as shown in the example below:

Example rat protein  

MKDLRFGRLKCYSLHLGILPLTVLVLVFFCFVCLSLRS QEWGHPGAVNRKAYPQPRVLTPTRTDVLVLTP WLAPIIWEGTFNIDILNEQFRLRNTTIGLTVFAIKKYVV FLKLFLETAEQHFMVGHKVIYYVFTDRPADV PQVPLGAGRRLVVLAVRNYTRWQDVSMHRMEIISHF SEQRFWHEVDYLVCGDVDMKFRDHVGVEILSALF GTLHPGFYRSRRESFTYERRPQSQAYIPWDQGDFY YMGAFFGGSVVEVHHLTKACHQAMVEDQDNGIEAV WHDESHLNKYLLYHKPTKVLSPEYMWDQQLLGWPS IMKKLRYVAVPKNHQAIRN




That's the gist of how to search for article and gene,
and that's all from us!

Thank you 😎

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